Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol |
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| Authors: | Nieves R.A. Ehrman C.I. Adney W.S. Elander R.T. Himmel M.E. |
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| Affiliation: | 1. National Renewable Energy Laboratory, Biotechnology Center for Fuels and Chemicals, Golden, CO, 80401, USA ; fax
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| Abstract: | Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-d-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-d-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger. |
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