An improved rapid assay of glycogen synthase |
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Authors: | Emma Villa-Moruzzi Teresa Locci-Cubeddu Ettore Bergamini |
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Affiliation: | Istituto di Patologia Generale, Via Roma 55, Scuola Medica, I-56100 Pisa, Italy |
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Abstract: | As an alternative to rapid filtration washing the glycogen free of any unreacted UDP-[14C]-glucose by ascending chromatography (ethanol:water, 2:1) can be used. This technique also makes the filter paper assay of glycogen synthase much faster: The samples are ready for liquid scintillation counting in 30 min. Among the other advantages offered by this procedure, we should also mention that blanks are very low, large volumes of ethanol can be saved, and the unreacted UDP-[14C]glucose can be recovered by elution and recycled (it migrates with the front of the solvent). |
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Keywords: | To whom reprint requests should be addressed. |
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