| 加拿大豌豆品种（系）抗白粉病表型和基因型鉴定 |
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| 引用本文： | 朱振东,付海宁,孙素丽,段灿星,杨晓明.加拿大豌豆品种（系）抗白粉病表型和基因型鉴定[J].植物遗传资源学报,2014,15(5):1028-1133. |
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| 作者姓名： | 朱振东 付海宁 孙素丽 段灿星 杨晓明 |
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| 作者单位： | 中国农业科学院作物科学研究所,甘肃农业大学生命科学技术学院，中国农业科学院作物科学研究所,中国农业科学院作物科学研究所,中国农业科学院作物科学研究所,甘肃农业大学生命科学技术学院, 甘肃农业科学院作物所 |
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| 基金项目： | 国家现代农业产业技术体系建设专项、作物种质资源保护子项目、国家自然科学基金项目（面上项目） |
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| 摘 要： | 对36个引自加拿大的豌豆品种(系)进行抗白粉病表型和标记基因型鉴定,明确了豌豆品种Cooper和Tara白粉病抗性等位基因。苗期接种了2个不同地理来源的豌豆白粉病菌分离物,32个品种(系)对2个分离物均表现为免疫;品系MP1818-2对云南白粉菌分离物EPYN免疫,但对北京分离物EPBJ感病;其余3个品种对2个分离物均感病。4个与豌豆抗白粉病基因er1连锁的SCAR标记将36个豌豆品种(系)区分为5个标记基因型。与野生型PsMLO1基因序列比较发现,豌豆品种Cooper和Tara的PsMLO1候选基因均在680 bp处发生C变G的单核苷酸突变。
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| 关 键 词： | 豌豆 白粉病 抗病性 标记基因型 er1 |
| 收稿时间： | 1/5/2014 12:00:00 AM |
| 修稿时间： | 3/2/2014 12:00:00 AM |
Phenotypic and Genotypic Identification of Powdery Mildew Resistance in 36 Pea Cultivars or Lines from Canada |
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| Zhu Zhendong,Fu Haining,Sun Suli,Duan Canxing and Yang Xiaoming.Phenotypic and Genotypic Identification of Powdery Mildew Resistance in 36 Pea Cultivars or Lines from Canada[J].Journal of Plant Genetic Resources,2014,15(5):1028-1133. |
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| Authors: | Zhu Zhendong Fu Haining Sun Suli Duan Canxing and Yang Xiaoming |
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| Institution: | FU Hai-ning;SUN Su-li;ZHU Zhen-dong;DUAN Can-xing;YANG Xiao-ming;College of Life Science and Technology,Gansu Agricultural University;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences /The National Key Facility for Crop Gene Resources and Genetic Improvement;Crop Institute,Gansu Academy of Agriculture Sciences; |
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| Abstract: | In this study, the phenotype and genotype of powdery mildew resistance in 36 pea cultivars or lines introduced from Canada were identified, and the allele at powdery mildew resistance gene er1 locus in cultivars Cooper and Tara were investigated. All tested cultivars or lines were inoculated by two powdery mildew isolates from different geographical origins in seedling stage, respectively. Thirty-two cultivars or lines were immune to the two isolates, and line MP1818-2 was immune to isolate EPYN and susceptible to isolate EPBJ, whereas 3 cultivars were susceptible to the two isolates. Five marker genotypes were identified in the 36 cultivars or lines by 4 SCAR markers linked to er1. Sequence analysis revealed that both PsMLO1 candidate gene in Cooper and Tara contained a point mutation which G replaced C of PsMLO1 gene at 680 bp site. |
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| Keywords: | Pisum sativum Powdery mildew Resistance Marker genotype er1 |
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