| 特早熟甘蓝型油菜异交率的测定 |
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| 引用本文： | 李开祥,杜德志. 特早熟甘蓝型油菜异交率的测定[J]. 植物遗传资源学报, 2014, 15(5): 1156-1160 |
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| 作者姓名： | 李开祥 杜德志 |
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| 作者单位： | 青海省农林科学院油菜所 |
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| 基金项目： | 国家油菜产业技术体系(CARS-13) |
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| 摘 要： | 利用SSR分子标记技术构建3个特早熟甘蓝型油菜恢复系的指纹图谱,筛选出这3个恢复系的共显性SSR标记,测定了这3个恢复系的异交率。结果表明,恢复系材料4395、3509、4152的异交率分别为46.02%、33.32%、18.12%,在P<0.01时呈极显著差异,说明这3个恢复系的异交差异明显,为确定这3个恢复系在综合杂交种中的比例提供依据。
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| 关 键 词： | 特早熟甘蓝型油菜 异交率 综合杂交种 SSR |
| 收稿时间： | 2013-11-30 |
| 修稿时间： | 2014-01-29 |
Determination of early maturing B.napus outcrossing rate |
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| LI Kai-xiang;DU De-zhi. Determination of early maturing B.napus outcrossing rate[J]. Journal of Plant Genetic Resources, 2014, 15(5): 1156-1160 |
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| Authors: | LI Kai-xiang DU De-zhi |
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| Affiliation: | LI Kai-xiang;DU De-zhi;Qinghai Academy of Agriculture and Forestry/ Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province/National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm; |
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| Abstract: | Abstract:Fingerprinting of three very early Brassica napus restorer lines by using SSR molecular markers . CO dominant SSR markers were found in the 3 early maturing B.napus restorer lines and their F1; Those primers were used to determine the outcrossing rate of these 3 restorer lines in fields. The results showed that the outcrossing rate of these restorer lines (4395, 3509, 4152 ) were respectively in 46.02%, 33.32%, 18.12%, there was a significant difference in p = 0.01, it illustrated that the outcrossing rate of three very early maturing B.napus restorer lines had differences; And It provided a basis that to determine the proportion of three very early Brassica napus restorer lines in Synthetic hybrids. |
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| Keywords: | Key words: outcrossing rate Brassica napus L. Synthetic hybrids SSR |
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