Approaches to the molecular cloning of protein-tyrosine phosphatases in insulin-sensitive tissues |
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Authors: | Barry J. Goldstein Wei-Ren Zhang Naotake Hashimoto C. Ronald Kahn |
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Affiliation: | (1) Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 02215 Boston, MA, USA;(2) Research Division, Joslin Diabetes Center, One Joslin Place, 02215 Boston, MA, USA |
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Abstract: | The intrinsic tyrosyl kinase activity of the insulin receptor is regulated by a balance between insulin-induced receptor autophosphorylation, which stimulates the receptor kinase, and enzymatic dephosphorylation of the receptor, which deactivates its kinase activity. The cellular protein-tyrosine phosphatase (PTPase) enzymes responsible for reversing the activated state of the insulin receptor have not been characterized. Our laboratory is interested in identifying and cloning the specific PTPase(s) that regulate the phosphorylation state of the insulin receptor. This chapter will summarize the design and results of our initial molecular cloning studies to identify specific PTPases in insulin-sensitive tissues that may have a potential physiological role in insulin action and clinical insulin resistance. |
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Keywords: | insulin action protein-tyrosine phosphatases insulin resistance protein phosphorylation insulin receptor |
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