Discrimination and consistency of five myosin ATPase stains in human normal and duchenne dystrophic muscle |
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Authors: | J. M. Lester D. I. Silber W. G. Bradley M. H. Cohen R. P. Hirsch J. F. Brenner |
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Affiliation: | (1) Image Analysis Laboratory, Tufts-New England Medical Center, 171 Harrison Avenue, Box 246, 02111 Boston, MA, USA;(2) Department of Neurology, Tufts-New England Medical Center, 171 Harrison Avenue, 02111 Boston, MA, USA;(3) Department of Ophthalmology, Tufts-New England Medical Center, 171 Harrison Avenue, 02111 Boston, MA, USA |
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Abstract: | Summary Limitations in the ability of the human visual system to assess accurately the relative staining densities of individual fibers in muscle tissue stained for myosin ATPase can complicate the objective evaluation of fiber type populations. In this study a novel approach is employed which utilizes human visual capabilities to provide accurate fiber classification. Using this approach, the ability of five ATPase staining techniques to discriminate fiber type categories in single samples of human normal and Duchenne dystrophic skeletal muscle is evaluated, as is the consistency of the fiber type classifications between stains. While no major discrepancies in fiber typing were observed in the sample of normal muscle, significant differences in classification, along with a decrease in the ability to discriminate fiber types were noted in the sample of Duchenne muscular dystrophy. For the most part, these discrepancies were resolved by a re-interpretation of the staining characteristics of fibers in one stain.This work was supported in part by NIH grant NS 15584, and by a grant from the Muscular Dystrophy Association |
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