| 用ERIC—PCR法研究番茄根际细菌群落结构变化 |
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| 引用本文： | 朱红惠 姚青 赵立平. 用ERIC—PCR法研究番茄根际细菌群落结构变化[J]. 微生物学通报, 2003, 30(4): 10-14 |
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| 作者姓名： | 朱红惠 姚青 赵立平 |
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| 作者单位： | [1]广东省微生物研究所，广州510070 [2]华南农业大学，广州510642 [3]上海交通大学，上海200240 |
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| 基金项目： | 国家自然科学基金资助项目(No. 30 0 0 0 0 0 6 )~~ |
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| 摘 要： | 采用单一碳源回收菌群的方法和ERIC—PCR方法相结合，检测番茄(Lycopersicon escu-lentum Mill．)根际接种转基因微生物E4(Enterobacteria cloacae)后的根际微生物的群落结构和多样性的变化。结果表明：采用单一碳源回收菌群和ERIC—PCR相结合的方法，可以准确、直观和清楚地检测到E4释放到环境中后对根际微生物的群落结构和种群数量的影响。这种单一碳源培养法与ERIC—PCR相结合的方法，将有可能成为一种研究环境微生物群落结构变化的常用方法。
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| 关 键 词： | 根际细菌 群落结构 单一碳源回收 ERIC—PCR |
| 文章编号： | 0253-2654(2003)04-0010-05 |
| 修稿时间： | 2002-09-02 |
FLUCTUATION OF BACTERIA COMMUNITY STRUCTURE IN TOMATO RHIZOSPHERE AS REVEALED BY ERIC-PCR |
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| ZHU Hong Hui YAO Qing ZHAO Li Pi ng. FLUCTUATION OF BACTERIA COMMUNITY STRUCTURE IN TOMATO RHIZOSPHERE AS REVEALED BY ERIC-PCR[J]. Microbiology China, 2003, 30(4): 10-14 |
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| Authors: | ZHU Hong Hui YAO Qing ZHAO Li Pi ng |
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| Affiliation: | ZHU Hong Hui 1 YAO Qing 2 ZHAO Li Pi ng 3 |
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| Abstract: | With an improved method of sole carbon source utilization patterns of bacteria combined with ERIC PCR, it was detected the fluctuation of bacterial c o mmunity structure and diversity in tomato (Lycopersicon esculentum Mill ) rhiz osphere, which was influenced by a genetic modified bacterium strain E4 (Enter obacteria cloacae) Results indicated that the influence of E4 releasing on t he b a cterial community structure and population could be accurately and clearly manif ested through this combined method It was suggested that the combined method w o uld become a routine method in the research of environmental microorganism commu nity structure |
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| Keywords: | Rhizosphere bacteria Community structure Sole carbon source utiliza tion patterns ERIC PCR |
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