A method for concentrating dilute solutions of macromolecules |
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Authors: | H.G. Vartak M.V. Rele M. Rao V.V. Deshpande |
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Affiliation: | Biochemistry Division, National Chemical Laboratory, Poona 411 008, India |
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Abstract: | A simple, inexpensive acrylate polymer which has a capacity to absorb 170 ml of water per g has been developed. It can be used to concentrate dilute solutions of macromolecules such as proteins, nucleic acids, and carbohydrates. The polymer absorbs only low-molecular-weight substances such as glucose, sucrose, and inorganic salts. It can replace the various conventional concentration methods. No special device or electricity is needed for the concentration. The inexpensive polymer, molded in the form of rods, can be very conveniently used as “disposable concentration sticks.” |
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Keywords: | carbohydrate concentration concentration of macromolecules dewatering of macromolecules DNA concentration polyacrylate gel protein concentration |
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