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Recovery of proteins on a milligram scale from polyacrylamide electrophoresis gels,exemplified by purification of a retinol-binding protein |
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| Authors: | Lars-Göran Öfverstedt Johan Sundelin Gunnar Johansson |
| Institution: | 1. Institute of Biochemistry, Biomedical Center, University of Uppsala, P.O. Box 576, S-751 23 Uppsala, Sweden;2. Department of Cell Research, The Wallenberg Laboratory, University of Uppsala, P.O. Box 562, S-751 22 Uppsala, Sweden |
| Abstract: | An apparatus suitable for the recovery of proteins from polyacrylamide gels on a milligram scale by displacement electrophoresis (isotachophoresis) is described along with a buffer system that is suitable for this purpose with most proteins. The technique is illustrated by the recovery of a protein from a 15% polyacrylamide gel. The recovery was almost quantitative and the eluted protein showed little contamination upon quantitative amino acid analysis and automatic Edman degradation. |
| Keywords: | preparative polyacrylamide gel electrophoresis elution technique protein recovery displacement electrophoresis isotachophoresis retinol-binding protein |
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