An affinity ligand for phospholipase A2 purification |
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Authors: | S Tahir RC Hider |
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Institution: | Department of Chemistry, University of Essex, Colchester, CO4 3SQ, United Kingdom |
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Abstract: | An alkyl ether analog of phosphatidylcholine was synthesized and used as a ligand to purify acid-extracted phospholipase A2 from bovine ileum smooth muscle by affinity chromatography in the presence of cholate. This ligand contains a primary amino group at the ω-position of the acyl chain in position 1 and so permits direct covalent coupling with the ester group of Affi-Gel-10. An endogenous membrane bound phospholipase A2 has been purified 32-fold in a good yield (70%) employing this ligand in an affinity chromatography step. |
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Keywords: | affinity chromatography |
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