An affinity ligand for phospholipase A2 purification

An alkyl ether analog of phosphatidylcholine was synthesized and used as a ligand to purify acid-extracted phospholipase A₂ from bovine ileum smooth muscle by affinity chromatography in the presence of cholate. This ligand contains a primary amino group at the ω-position of the acyl chain in position 1 and so permits direct covalent coupling with the ester group of Affi-Gel-10. An endogenous membrane bound phospholipase A₂ has been purified 32-fold in a good yield (70%) employing this ligand in an affinity chromatography step.