Single-step,rapid separation of acidic and basic isoenzymes from commercial horseradish peroxidase |
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| Authors: | Aurel Popa Wagner Eugenia Psarrou Lieselotte Popa Wagner |
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| Affiliation: | Faculty of Medicine, Department of Biochemistry, Str. Petru Rares No 4, Craiova 1100, Romania |
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| Abstract: | A simple and sensitive high-performance liquid chromatography method with electrochemical detector is described for the determination of free 3-methoxy-4-hydroxyphenylacetic acid (HVA) in human plasma. The method does not involve any extraction, is specific and reproducible, and has the potential to measure serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) simultaneously. The plasma concentration of free HVA in eight normal, healthy adult volunteers was 10.9 ± 4.6 (mean ± SD). In a preliminary study, in one schizophrenic patient the plasma HVA increased twofold after neuroleptic treatment. |
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| Keywords: | To whom correspondence should be addressed: Str. Viitorului No 61 Medias 3125 Romania. |
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