1. Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20205 USA;2. The Hematology Service, Clinical Center, National Institutes of Health, Bethesda, Maryland 20205 USA
Abstract:
The carbohydrate structure of human thrombin has been determined by direct probe mass spectrometry of the oligosaccharides released by trifluoroacetolysis from the asialo glycoprotein. The free oligosaccharides were studied as permethylated and N-trifluoroacetylated oligosaccharide alditols. The structure was confirmed by sequential exoglycosidase digestion of intact thrombin and sugar and methylation analysis of the oligosaccharides by gas-liquid chromatography-mass spectrometry. The results indicate the following structure:
