| 酶联免疫吸附试验定量检测血清肝素酶方法的建立及应用 |
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| 引用本文： | 田曙光,虞立霞,李素波,高红伟,王颖丽,宫锋. 酶联免疫吸附试验定量检测血清肝素酶方法的建立及应用[J]. 生物技术通讯, 2009, 20(4): 545-547. DOI: 10.3969/j.issn.1009-0002.2009.04.026 |
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| 作者姓名： | 田曙光 虞立霞 李素波 高红伟 王颖丽 宫锋 |
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| 作者单位： | 军事医学科学院,野战输血研究所,北京,100850 |
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| 基金项目： | 国家科技支撑计划重大项目 |
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| 摘 要： | 目的：建立一种简便、微创的血清肝素酶酶联免疫吸附（ELISA）定量检测方法，并对肿瘤患者和正常人血清肝素酶进行比较，初步探讨血清肝素酶水平与肿瘤发生、发展的关系。方法：选择鼠抗人肝素酶单克隆抗体和兔抗人肝素酶多克隆抗体，建立双抗夹心ELISA检测方法，并利用此方法检测健康献血者和肿瘤患者血清中的肝素酶水平。结果：组内数据稳定，可重复；肿瘤患者血清中肝素酶D450nm值高于健康献血者。结论：所建立的血清肝素酶ELISA检测方法灵敏、高效，可用于肿瘤的辅助诊断。
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| 关 键 词： | 肿瘤 肝素酶 双抗夹心酶联免疫吸附试验 |
Establishment and Application of ELISA Method for Detecting of Human Serum Heparanase |
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| TIAN Shu-Guaag,YU Li-Xia,LI Su-Bo,GAO Hong-Wei,WANG Ying-Li,GONG Feng. Establishment and Application of ELISA Method for Detecting of Human Serum Heparanase[J]. Letters in Biotechnology, 2009, 20(4): 545-547. DOI: 10.3969/j.issn.1009-0002.2009.04.026 |
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| Authors: | TIAN Shu-Guaag YU Li-Xia LI Su-Bo GAO Hong-Wei WANG Ying-Li GONG Feng |
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| Affiliation: | (Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China) |
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| Abstract: | Objective： To establish a simple, minimally invasive serum heparanase quantitative ELISA detection methods,and preliminary study the relations of the serum heparanase levels and tumor occurrence and development. Methods： The mouse anti-human heparanase monoclonal antibody and rabbit anti-human heparanase polyclonal antibody were used as the first antibodies of an antibody-sandwich ELISA. Heparanase levels of healthy blood donors and cancer patients serum were detected using this ELISA method. Results： D450nm values of tumor patients are significantly higher than those of healthy blood donors. Conclusion： ELISA method for detecting of human serum heparanase is sensitive and high-performance, it can be used for tumor diagnosis. |
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| Keywords: | tumor heparanase antibody-sandwich ELISA |
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