Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7 |
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Authors: | Suzuki Masahiro Kondo Fumio Ito Yuko Matsumoto Masakado Hata Mami Oka Hisao Takahashi Masao Sakae Kenji |
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Affiliation: | Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan. masahiro_4_suzuki@pref.aichi.lg.jp |
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Abstract: | We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product. |
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Keywords: | Shiga toxin 1 variant IS1203 Shiga toxin-producing Eschericia coli O157 |
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