Effects of theophylline on chromosomal breakage and sister-chromatid exchange |
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Authors: | P Day Z Shalaby M M Cohen S S Wasserman S Schwartz |
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Affiliation: | Department of Obstetrics/Gynecology, University of Maryland, School of Medicine, Baltimore 21201. |
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Abstract: | Frequencies of both sister-chromatid exchange (SCE) and chromosomal breakage (CB) were studied in the lymphocytes of normal individuals (10 and 7 individuals respectively). The cells were exposed in vitro to 3 different concentrations of theophylline (1, 10 and 100 micrograms/ml). A significant concentration effect of the drug was demonstrated for both SCEs and CB. Utilizing a Dunnett's test for individual comparisons, the 10 and 100 micrograms/ml concentrations both demonstrated a significant elevation of SCEs and CB compared to the untreated control cultures. This study suggests that in vitro concentrations of theophylline equal to or greater than 10 micrograms/ml, corresponding to serum levels attained during therapy, increase the frequency of SCEs and chromosome breakage in human lymphocytes. |
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