Preparation and characterization of neurotoxic tau oligomers |
| |
Authors: | Lasagna-Reeves Cristian A Castillo-Carranza Diana L Guerrero-Muoz Marcos J Jackson George R Kayed Rakez |
| |
Institution: | The George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, Galveston, Texas 77555-1045, United States. |
| |
Abstract: | Tau aggregation is a pathological hallmark of Alzheimer's disease, Parkinson's disease, and many other neurodegenerative disorders known as tauopathies. Tau aggregates take on many forms, and their formation is a multistage process with intermediate stages. Recently, tau oligomers have emerged as the pathogenic species in tauopathies and a possible mediator of amyloid-β toxicity in Alzheimer's disease. Here, we use a novel, physiologically relevant method (oligomer cross-seeding) to prepare homogeneous populations of tau oligomers and characterize these oligomers in vitro. We show that both Aβ and α-synuclein oligomers induce tau aggregation and the formation of β-sheet-rich neurotoxic tau oligomers. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|