| Abstract: | Four genotypes of Brassica nigra were tested for their abilityto produce somatic embryos in vitro. Seedling-derived hypocotylexplants cultured in MS medium with p-chlorophenoxyacetic acid, -naphthaleneacetic acid and adenine gave rise to embryos thatcould germinate into seedlings with a high frequency on transferto medium containing benzylaminopurine riboside and p-chlorophenoxyaceticacid. Ebryogenesis was highest in leaf explants followed bystem and hypocotyls. Comparison of the embryogenic responseof hypocotyl segments differing in age indicated an increasein the frequency of response with increasing age of the explants.However, germination of embryos into seedlings declined withincreasing age of the explant. Embryogenesis was higher in MSmedium compared to five other media with similar growth regulatorcomposition. Genotypic differences exist for frequency of embryogenesisand subsequent maturation into seedlings. Key words: Brassica nigra, somatic embryogenesis, growth regulators, plant regeneration |
