Potential target gene and protein interaction ofPimpinella brachycarpa HD-Zip protein, Phz4 by yeast two-hybrid system |
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| Authors: | Sanghyun Lim Yong -Hwan Moon Jung -Il Cho Jong Chan Woo Kwang -Woong Lee |
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| Institution: | (1) Department of Biology, Seoul National University, 151-742 Seoul, Korea;(2) Department of Plant and Microbial Biology, University of California, 94720-3012 Berkeley, California, USA |
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| Abstract: | The yeast two-hybrid system was used to investigate dimerization between proteins ofPhz2 andPhz4 clones of the homeodomain-leucine zipper family which were obtained by screening aPimpinella brachycarpa shoot-tip cDNA library. Assays showed that Phz4 formed a homo rather than a heterodimer with Phz2. In addition, we isolated
cDNA clones,Phyb1, Phyb2, andPhyb3, that encode proteins interacting with Phz4. Although Phyb1 is not a HD-Zip protein, the activity of interaction between
Phyb1 and Phz4 was, surprisingly, stronger than that of the homodimerization of Phz4. The analysis of interacting parts indicated
that from 1 bp to 466 bp of Phyb1, there was no interaction with Phz4, but from 467 bp to 593 bp, interactions were found
with the N-terminal and C-terminal regions, except for HD-Zip of Phz4. This region ofPhyb1 contained a nuclear localization signal. DNA-binding analysis showed that the Phz4 HD-Zip domain recognized the T(C/G)ATTG]
core sequence and the region containing the TCATTG] motif, which is, in itself, a promoter in vitro. |
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| Keywords: | homeodomain leucine zipper protein-protein interaction target gene |
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