Impact of UV-B Radiation on Thylakoid Membrane and Fatty Acid Profile of Spirulina platensis |
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Authors: | Radha Gupta Pratiksha Bhadauriya Vinay Singh Chauhan Prakash Singh Bisen |
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Affiliation: | (1) Department of Biotechnology, Madhav Institute of Technology & Science, Race Course Road, Gwalior, 474005, India;(2) Department of Biotechnology, J. C. Bose Institute of Life Sciences, Bundelkhand University, Kanpur Road, Jhansi, 284218, India |
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Abstract: | Ultraviolet-B (UV-B) radiation to thylakoid membrane and fatty acid profile has been investigated in cyanobacterium, Spirulina platensis. The thylakoid membrane was isolated by mechanical disruption of the freeze-dried and lysozyme-treated cells followed by differential density gradient centrifugation and morphological variations were examined. UV radiation distorted the membrane on the outer side with reduced chlorophyll a (chl a) content compared to its untreated counterpart. Liquid chromatography-mass spectrometry (LC-MS) was used for characterization of chl a of the thylakoid membrane. UV-B exposure resulted in alterations in the pigment-protein complexes 47 kDa and 43 kDa. Furthermore, 94 kDa and 20 kDa protein appeared in UV-B-exposed thylakoid membrane of S. platensis. The composition of fatty acid in response to UV-B radiation was detected by gas chromatography–mass spectrometry having 23.5% saturated fatty acid (SFA), 76.4% monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA). In contrast to its UV-B-untreated counterpart, SFA was 46.6%, and MUFA and PUFA were 53.3%. Our findings suggest that UV-B radiation not only affects membrane morphology and its protein profile but also reduces saturated fatty acid and increases unsaturated fatty acids in S. platensis. |
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