| Abstract: | Serotonin depolarized Aplysia buccal motoneuron B16. The response could be obtained in high-magnesium/low-calcium medium, indicating a direct effect on B16 and supporting a putative monosynaptic input to B16 from the serotonergic metacerebral neurons. Similar depolarizing effects in high-magnesium/low-calcium medium were obtained in response to 8-bromo cyclic AMP, isobutylmethylxanthine, theophylline and forskolin. Tolbutamide, a putative inhibitor of cyclic AMP-dependent protein kinase, blocked or reversed responses of B16 to egg-laying hormone containing extracts and to serotonin. Serotonin and forskolin significantly increased the cyclic AMP content of buccal ganglia, whereas egg-laying hormone-containing extracts did not. |
