Superoxide dismutase activity in salt stressed wheat seedlings |
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Authors: | Füsun? Eyido?an Email author" target="_blank">Hüseyin?Avni??ktemEmail author Meral?Yücel |
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Institution: | (1) Department of Biology, Middle East Technical University, 06531 Ankara, Turkey;(2) Present address: Education Faculty, Baskent University, Ankara, Turkey |
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Abstract: | We investigated the effect of salt stress on enzymatic activity of superoxide dismutase (SOD) isozymes in shoot and root tissues
of salt tolerant and sensitive wheat (Triticum aestivum L. and Triticum durum Defs.) cultivars. Ten day old seedlings were subjected to 0.7 M NaCl stress for 3 and 5 days. Seedlings treated in the same
manner without salt stress served as controls. Activity of SOD isozymes in root and shoot extracts was determined by activity
staining of native polyacrylamide gels. In both shoot and root extracts of examined cultivars two isozymes of SOD, namely
MnSOD and Cu/ZnSOD were identified. Cu/ZnSOD activity comprised 90 % of total SOD activity in both root and shoot tissues.
Salt stress caused 1–1.5 fold increase in MnSOD activity of shoots in tolerant cultivars when compared with non-stressed controls.
Under stress conditions, compared to controls all cultivars exhibited reduced MnSOD activity in root tissues. Cu/ZnSOD activity,
on the other hand, was remarkably enhanced (3–4 fold) in root extracts of the tolerant cultivars, whereas it was reduced in
the sensitive ones. |
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Keywords: | NaCl salt stress SOD superoxide dismutase wheat |
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