Diffusion measurements of water, ubiquinone and lipid bilayer inside a cylindrical nanoporous support: A stimulated echo pulsed-field gradient MAS-NMR investigation |
| |
Authors: | Olivier Wattraint Catherine Sarazin |
| |
Affiliation: | Unité de Génie Enzymatique et Cellulaire, UMR 6022 du CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens cedex, France |
| |
Abstract: | Stimulated echo pulsed-field gradient 1H magic angle spinning NMR has been used to investigate the mobility of water, ubiquinone and tethered phospholipids, components of a biomimetic model membrane. The diffusion constant of water corresponds to an isotropic motion in a cylinder. When the lipid bilayer is obtained after the fusion of small unilamellar vesicles, the extracted value of lipid diffusion indicates unrestricted motion. The cylindrical arrangement of the lipids permits a simplification of data analysis since the normal bilayer is perpendicular to the gradient axis. This feature leads to a linear relation between the logarithm of the attenuation of the signal intensity and a factor depending on the gradient strength, for lipids covering the inner wall of aluminium oxide nanopores as well as for lipids adsorbed on a polymer sheet rolled into a cylinder. The effect of the bilayer formation on water diffusion has also been observed. The lateral diffusion coefficient of ubiquinone is in the same order of magnitude as the lipid lateral diffusion coefficient, in agreement with its localization within the bilayer. |
| |
Keywords: | AAO, anodic aluminum oxide DOPE, 1,2-dioleoyl-sn glycero-3-phosphatidylethanolamine EggPC, egg yolk L-α-phosphatidylcholine HR-MAS, high resolution magic angle spinning MLVs, multilamellar vesicles NMR, nuclear magnetic resonance PEG, polyethylene glycol NHS, N-hydrosuccinimide PET, polyethylene terephtalate PFG-STE, pulsed-field gradient stimulated echo POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine SUVs, small unilamellar vesicles |
本文献已被 ScienceDirect 等数据库收录! |
|