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Melatonin strongly interacts with zwitterionic model membranes—evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry
Authors:Feride Severcan  Ipek Sahin
Institution:a Department of Biology, Middle East Technical University, 06531 Ankara, Turkey
b Department of Physics, Faculty of Science, Ege University, 35100 Bornova-?zmir, Turkey
Abstract:Interactions of melatonin with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and melatonin concentration (1-30 mol%) by using two noninvasive techniques, namely Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). The investigation of the C-H, C6-point double bond; length half of m-dashO, and PO2 antisymmetric double stretching modes in FTIR spectra and DSC studies reveal that melatonin changes the physical properties of the DPPC bilayers by decreasing the main phase transition temperature, abolishing the pretransition, ordering the system in the gel phase, and increasing the dynamics of the system both in the gel and liquid crystalline phases. It also causes significant decrease in the wavenumber for the C6-point double bond; length half of m-dashO stretching and PO2 antisymmetric double bond stretching bands, which indicates strong hydrogen bonding The results imply that melatonin locates in the interfacial region of the membrane. Furthermore, in the DSC curve, more than one signal is observed at high melatonin concentrations (24 and 30 mol%), which indicates melatonin-induced phase separation in DPPC membranes.
Keywords:Melatonin  Dipalmitoyl phosphatidylcholine  Membrane  Liposome  Fourier transform infrared  Differential scanning calorimetry  Phase separation
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