Biological and polymeric self-assembled hybrid systems: Structure and properties of thylakoid/polyelectrolyte complexes |
| |
Authors: | A.A. Dementiev A.N. Tikhonov |
| |
Affiliation: | Department of Biophysics, Faculty of Physics, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia |
| |
Abstract: | A novel hybrid system composed of biological components and synthetic polymer, thylakoid/polycation complex, has been formed and studied. Effects of complex formation on the structure, electrostatics and functioning of thylakoid membranes have been examined. Thylakoids from bean leaves were used to form complexes with polycation polyallylamine hydrochloride (PAAH) in two systems: (i) thylakoid/polycation complexes formed in an aqueous bulk phase, and (ii) immobilized thylakoid/polycation planar complexes. Immobilized on a solid substrate surface, thylakoid/polycation complexes were prepared using layer-by-layer stepwise alternate adsorption technique, i.e., via the sequential alternate adsorption of thylakoids and polycation molecules. The morphology of built up structures was investigated by scanning electron microscopy. Light-induced electron transport in chloroplasts was studied by the electron paramagnetic resonance (EPR) method. Spin probe technique was employed to study the structural and electrostatic characteristics of thylakoid membranes. We have found that efficiency of light-induced electron transport in thylakoid membranes and membrane structure were not changed noticeably by PAAH binding to thylakoids in a wide range of PAAH concentrations. The data obtained indicate the physiologically-soft character of polycation interactions with thylakoid membranes and demonstrate effectiveness of interfacial self-assembly approach to fabrication of complex planar functional nanostructures from biological components and synthetic polymers. |
| |
Keywords: | EPR, electron paramagnetic resonance CAT9, N,N-dimethyl-N-nonyl-tempoylammonium bromide CTAB, cetyltrimethylammonium bromide PAAH, polyallylamine hydrochloride PEI, polyethylenimine PSS, polystyrene sulfonate PSI and PSII, photosystems I and II, respectively S.E.M., scanning electron microscopy 5-SASL, 5-doxylstearate 16-MeSASL, 16-doxylstearate methyl ester |
本文献已被 ScienceDirect 等数据库收录! |
|