Electrophysiological and molecular identification of hepatocellular volume-activated K channels

Although K⁺ channels are essential for hepatocellular function, it is not known which channels are involved in the regulatory volume decrease (RVD) in these cells. We have used a combination of electrophysiological and molecular approaches to describe the potential candidates for these channels. The dialysis of short-term cultured rat hepatocytes with a hypotonic solution containing high K⁺ and low Cl⁻ concentration caused the slow activation of an outward, time-independent current under whole-cell configuration of the patch electrode voltage clamp. The reversal potential of this current suggested that K⁺ was the primary charge carrier. The swelling-induced K⁺ current (I_Kvol) occurred in the absence of Ca²⁺ and was inhibited with 1 μM Ca²⁺ in the pipette solution. The activation of I_Kvol required both Mg²⁺ and ATP and an increasing concentration of Mg-ATP from 0.25 through 0.5 to 0.9 mM activated I_Kvol increasingly faster and to a larger extent. The KCNQ1 inhibitor chromanol 293B reversibly depressed I_Kvol with an IC₅₀ of 26 μM. RT-PCR detected the expression of members of the KCNQ family from KCNQ1 to KCNQ5 and of the accessory proteins KCNE1 to KCNE3 in the rat hepatocytes, but not KCNQ2 and KCNE2 in human liver. Western blotting showed KCNE3 expression in a plasma membrane-enriched fraction from rat hepatocytes. The results suggest that KCNQ1, probably with KCNE2 or KCNE3 as its accessory unit, provides a significant fraction of I_Kvol in rat hepatocytes.