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Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme
Authors:Takaomi Fushimi  Masaaki Umeda  Tetsuo Shimazaki  Atsushi Kato  Kinya Toriyama  Hirofumi Uchimiya
Affiliation:(1) Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Botany, Hokkaido University, 060 Sapporo, Japan;(3) Faculty of Agriculture, Tohoku University, 981 Sendai, Japan;(4) Shiraoka Research Station of Biological Science, Nissan Chemical Industries, Ltd., Minamisailama, 349-02 Saitama, Japan
Abstract:We have isolated a rice cDNA clone that is homologous to the gene for the maize NADP-dependent malic enzyme (EC 1.1.1.40; NADP-ME). The deduced amino acid sequence coded for by the cDNA indicates a high level of homology to chloroplast type NADP-ME, including a transit peptide with pronounced hydrophobic properties at the amino terminus. Northern blot analysis indicates that the expression of this gene is regulated by external stress such as submergence.
Keywords:rice (Oriza sativa L.)  cDNA and NADP-malic enzyme
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