Response of antioxidant systems in oxygen deprived suspension cultures of rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) |
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Authors: | Revandy Iskandar Damanik Mohd Razi Ismail Zulkifli Shamsuddin Sariam Othman Abd Mohd Zain Mahmood Maziah |
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Institution: | (1) Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;(2) Institute of Tropicial Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;(3) Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;(4) Rice and Industrial Crop Research Centre MARDI Head Quarters, 50774 Kuala Lumpur, Malaysia;(5) Department of Agrotechnology, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia; |
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Abstract: | The effect of oxygen deprivation (anoxia) on the antioxidant system in suspension culture of anoxia-intolerant Malaysian rice
mutants cells was examined. Abiotic stresses have been reported to adversely affect cell division, damage cellular and organelle
membranes. The signaling defense mechanisms, such as molecular and biochemical aspects responding to stress have been proven
to be very complex, and still largely untapped. The objective of this study was to determine the potential involvement of
activated oxygen species, such as superoxide dismutase, catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase
which occur in cells of rice plants exposed to anoxia stress in two Malaysian rice mutants, MR219-4 and MR219-9, and rice
cultivar FR13A which is known to be tolerant to anoxia stress during 5–30 days of exposure. The antioxidative enzymes were
decreased for MR219-4 and MR219-9 mutants for CAT and APX activities, and increased in FR13A cultivar starting at 20 days
in suspension culture compared to that of control. CAT and APX activities were maintained higher in anoxia condition for all
mutants and cultivar. These findings suggested that anoxia stress in suspension cultures induced the level of H2O2 to toxic levels. |
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