Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using <Emphasis Type="Italic">Cupriavidus necator</Emphasis> |
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| Authors: | José Geraldo da Cruz Pradella Jaciane Lutz Ienczak Cecília Romero Delgado Marilda Keico Taciro |
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| Institution: | 1.Laboratório Nacional de Ciência e Tecnologia do Bioetanol,Centro Nacional de Pesquisa em Energia e Materiais,Campinas,Brazil;2.Laboratório de Biotecnologia Industrial,Instituto de Pesquisas Tecnológicas do Estado de S?o Paulo,S?o Paulo,Brazil;3.Departamento de Microbiologia,Instituto de Ciências Biomédicas, Universidade de S?o Paulo,S?o Paulo,Brazil |
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| Abstract: | Poly(3-hydroxybutyrate) (PHB) biosynthesis from soybean oil by Cupriavidus necator was studied using a bench scale bioreactor. The highest cell concentration (83 g l−1) was achieved using soybean oil at 40 g l−1 and a pulse of the same concentration. The PHB content was 81% (w/w), PHB productivity was 2.5 g l−1 h−1, and the calculated Yp/s value was 0.85 g g−1. Growth limitation and the onset of PHB biosynthesis took place due to exhaustion of P, and probably also Cu, Ca, and Fe. |
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