Growth of separated and recombined neonatal rat uterine luminal epithelium and stroma on extracellular matrix: Effects of in vivo tamoxifen exposure |
| |
Authors: | William S Branham Beverly D Lyn-Cook Annette Andrews Daniel M Sheehan |
| |
Institution: | (1) Division of Reproductive and Developmental Toxicology, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(2) Division of Nutritional Toxicology, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(3) Pathology Associates, Inc., National Center for Toxicological Research, 72079 Jefferson, Arkansas;(4) Department of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(5) Departments of Biochemistry and Pharmacology, University of Arkansas for Medical Sciences, 72201 Little Rock, Arkansas |
| |
Abstract: | Summary We have developed a system for serum-free culture of separated uterine epithelium and stroma from 11-day-old rats recombined
on extracellular matrix extracted from Englebreth-Holm-Swarm tumors. Epithelium grew and, after 2 days in culture, developed
into luminal epithelial spheres (LES) surrounding a fluid-filled lumen. Individual LES cells maintained epithelial cell characteristics
such as basally located nuclei, apical microvilli (oriented toward the lumen), lateral membranes with interdigitations and
desmosomes, secretory Golgi complexes, and abundant mitochondria and rough endoplasmic reticulum. Secretory vesicles were
ubiquitous throughout the luminal fluid. Addition of 17β-estradiol to the growth medium increased the number and longevity of the LES. Prior exposure of uteri to tamoxifen via s.c.
injection in vivo on postnatal Days 1 to 5 reduced or completely inhibited formation of LES in vitro. These effects occurred
regardless of whether the stromal or epithelial component of the recombinant tissue was exposed to tamoxifen. These data suggest
a directive property of neonatal stroma in culture resulting in the formation of highly secretory spherical epithelial structures
completely enclosing a lumen. LES formation is responsive to both estrogen (positive response) and antiestrogen (negative
response). |
| |
Keywords: | uterus epithelium stroma extracellular matrix differentiation antiestrogen |
本文献已被 SpringerLink 等数据库收录! |
|