cDNA cloning and sequencing for the import precursor of subunit B in H(+)-ATP synthase from rat mitochondria |
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Authors: | C Tsurumi Y Yoshihara F Osaka F Yamada I Tani T Higuti M Shimizu K Oeda H Ohkawa H Toda |
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Affiliation: | Faculty of Pharmaceutical Sciences, University of Tokushima, Japan. |
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Abstract: | The nucleotide sequence of the import precursor of subunit b of rat liver H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 1,124 nucleotides including a coding region for the import precursor of subunit b and noncoding regions of both the 5'- and 3'-sides. The import precursor of subunit b and its mature polypeptide deduced from the open reading frame consisted of 256 and 214 amino acid residues with a molecular weight of 28,867 and 24,628, respectively. The presequence of 42 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix. |
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