Chimeric gene expression using maize intron in cultured cells of breadwheat |
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Authors: | J H Oard D Paige J Dvorak |
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Institution: | (1) Department of Agronomy and Range Science, University of California-Davis, 95616 Davis, CA, USA |
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Abstract: | High voltage electrical pulses were used to introduce the CAT reporter gene into cultured protoplasts of breadwheat,Triticum aestivum. Four DNA constructs harboring the CAT gene and the 35S or mannipine synthase promoter were tested for levels of CAT activity 40–45 hr after electroporation of protoplasts. One construct, containing a maize intron sequence between 35S and CAT sequences, conferred 30 to 185 fold greater CAT activity over the other three constructs. Data from these experiments suggest that a maize intron or sequences with similar effects may be required in DNA constructs for efficient heterologous gene expression in cultured cells of breadwheat.Abbreviations CAT
Chloramphenicol acetyl transferase
- NPT II
neomycin phosphotransferase
- 35S
the 35S promoter of Cauliflower Mosaic Virus
- PEG
Polyethylene glycol
- MES
2-N-morpholino] ethanesulfonic acid |
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Keywords: | |
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