草莓拟盘多毛孢叶斑病的病原菌

于2013年在北京市房山区发现了一种新的草莓叶部病害,为明确引起该病害的病原菌种类,采用组织分离法对病叶进行了病原菌的分离,经纯化得到一株病原菌CMF4。通过室内人工接种对该菌株的致病性和寄主范围进行测定,发现该菌株可以导致草莓叶片坏死和果实腐烂,可引起有伤供试植物牡丹、海棠、芍药、杏、樱桃、桃和月季发病,但不能无伤侵入,对人工接种发病的植株进行病原菌再分离可得到原接种病原菌。采用rDNA-ITS序列分析方法并结合该病原菌的形态特征进行鉴定,发现引起该病害的病原菌为棒孢拟盘多毛孢Pestalotiopsis clavispora。这是棒孢拟盘多毛孢所致草莓叶斑病在国内的首次报道。

Pestalotiopsis clavispora causing leaf spot on strawberry

A new leaf spot disease was observed on strawberry Fragaria ananassa in Fangshan District, Beijing in 2013. Isolation of the pathogen was made from symptomatic tissues and isolate CMF4 was obtained. The pathogenicity and host range was examined by inoculation in laboratory. The generated strain CMF4 was able to infect the damaged leaves and fruits of strawberry. Its host range test showed that it could infect the damaged leaves of peony Paeonia suffruticosa, Chinese flowering crabapple Malus spectabilis, Chinese herbaceous peony P. lactiflora, apricot Armeniaca vulgaris, cherry Cerasus pseudocerasus, peach Amygdalus persica and Chinese rose Rosa chinensis, but failed to infect their non-damaged leaves, and the similar fungus could be isolated from these diseased plants. Based on rDNA-internal transcribed spacer (ITS) analysis and morphological characters, the pathogen was identified as Pestalotiopsis clavispora. The pathogen was firstly reported in China responsible for Pestalotiopsis leaf spot of strawberry.