Determination of the cross-points of rat liver peroxisomes,peroxisomal core and the core components by cross-partition |
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Authors: | Shuichi Horie Hidemi Ishii Hiroyuki Nakazawa Tetsuya Suga Hirotake Orii |
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Affiliation: | 1. Department of Clinical Biochemistry, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan;2. Department of Radiology and Nuclear Medicine, Tokyo Metropolitan Institute of Medical Science, 6-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 Japan |
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Abstract: | The cross-points of rat liver peroxisomes, peroxisomal core and the core components were determined by means of cross-partition in two phase systems. The partitions were carried out in the systems containing 6% (w/w) Dextran T 500 and 6% (w/w) polyethyleneglycol 4000 in sodium salts. The same crosspoint, pH 5.6, was obtained in peroxisomal marker enzymes in light mitochondrial fraction of liver homogenate, such as catalase, d-amino acid oxidase and urate oxidase. The cross-point as determined by cross-partition of purified peroxisomal core was 6.7. The cross-points of urate oxidase and framework protein fractions obtained by alkali treatment on the purified core were 7.8 and 4.2, respectively, and the ratio of the proteins of urate oxidase to framework protein was 2:1. The theoretical value of cross-point of the core calculated from the relationship between the cross-point and protein ratio of each component of the core coincided with the experimental value obtained by this method. |
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Keywords: | Peroxisome Core component Cross-partition (Rat liver) |
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