Presence of IL-1- and TNF-like molecules in Galleria mellonella (Lepidoptera) haemocytes and in an insect cell line Fromestigmene acraea (Lepidoptera). |
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| Authors: | D Wittwer A Franchini E Ottaviani A Wiesner |
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| Institution: | 1. Institut für Zoologie, Freie Universität Berlin, 14195, Berlin, Germany;2. Dipartimento di Biologia Animale, Università di Modena, 41100, Modena, Italy;1. The State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400716, China;2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agricultural, Southwest University, Beibei, Chongqing 400716, China;3. Laboratory of Animal Biology, Chongqing Normal University, Chongqing 400047, China;4. Research Laboratory Center, Chongqing Three Gorges Medical College, Chongqing 404120, China;1. School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia Autonomous Region, 014010, China;2. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, 222005, China;3. College of Marine Science and Engineering, Nanjing Normal University, Nanjing, 210046, China;4. Library, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia Autonomous Region, 014010, China;1. Department of Infectious Diseases, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark;2. Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;3. Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark;4. Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark;5. Department of Clinical Medicine, Aalborg University, Aalborg, Denmark;6. Staphylococcus Laboratory, Statens Serum Institut, Copenhagen, Denmark;7. Bristol-Myers Squibb, Virum, Denmark;8. Clinical Research Centre, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark |
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| Abstract: | In this study the authors give immunocytochemical evidence for the presence of interleukin (IL)-1alpha- and tumour necrosis factor (TNF) alpha-like molecules in the haemocytes of last instar larvae from the greater wax moth Galleria mellonella. Similar results are demonstrated in a continuous haemocyte line (BTI-EA-1174-A) from the salt marsh caterpillar Estigmene acraea. In Galleria mellonella larvae granular cells show a strong positive reaction with both primary antibodies, whereas plasmatocytes are stained to a lesser extent. Cell line haemocytes also react positively with both antibodies. After activating the cells with lipopolysaccharide (LPS) staining of Estigmene acraea cells is decreased, whereas Galleria mellonella haemocytes show no visible reaction in comparison to non-activated cells. |
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