华南忍冬组织培养的无菌体系建立

以广西山银花的种植品种华南忍冬带腋芽茎段为外植体建立无菌体系。结果表明,较适宜的灭菌处理为外植体用75%酒精处理25s,再用0.1%升汞溶液(加吐温80)处理9min,污染率最低为6.7 %;初代培养腋芽萌发较适宜的培养基为MS+6-BA2.0mg/L,萌发率63.3%,比CK组的高33.3%;继代周期为30d,继代增殖较适宜培养基为MS+6-BA1.7mg/L,芽比较粗壮,平均每丛丛芽增加芽数为10.7个,比CK组高9.5个。

Tissue culture and rapid propagation of Lonicera confuse

The stems with axillary buds of Guangxi Lonicera confusa were taken as explants to build aseptic system. The results showed that the suitable disposal of sterilization was using 75% alcohol to dispose for 25 s firstly,and then using 0.1% HgCl2(with Polysor6-BAte 80) to dispose for 9min,the minimum rate of pollution was 6.7%;the suitable initial medium to germinate auxiliary buds was MS supplemented with 6-BA1.7 mg/L,germinative rate was 63.3% which was higher than CK 33.3%;the period of subculture was 30 d,the suitable subculture proliferous medium was MS supplemented with 6-BA1.7 mg/L,the buds were strong,and the average of the proliferous buds were 10.7 which was more than CK 9.5.