Alternaria Brassicae Induced Changes in the Activity of Cell Wall Degrading Enzymes in Leaves of Brassica Juncea |
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| Authors: | S. Garg K. Dhawan H.K.L. Chawla H.S. Nainawatee |
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| Affiliation: | (1) Departments of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar -, 125 004, India;(2) Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar -, 125 004, India |
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| Abstract: | After inoculation of Brassica juncea leaves with Alternaria brassicae, activities of the cell wall degrading enzymes, polygalacturonase (EC 3.2.1.15) and cellulase (EC 3.2.1.4) decreased in leaf blight resistant cultivar RC-781 and increased in the susceptible cultivar Varuna upto 3 d. In the leaves of both the cultivars 11 poly-peptides were observed in the absence of A. brassicae inoculation. After inoculation in the resistant cultivar RC-781 there was no change in the polypeptide pattern, while in the susceptible cultivar Varuna, four polypeptides (43.7 to 58.8 kDa) disappeared only at 3rd day after inoculation. This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | cellulase polygalacturonase protein electrophoresis |
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