Changes in endogenous cytokinins of celery (Apium graveolens L.) seeds following an osmotic priming or growth regulator soak treatment |
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Authors: | Tudor H Thomas |
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Institution: | (1) Plant Physiology Section, National Vegetable Research Station, CV35 9EF Wellesbourne, Warwick, UK |
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Abstract: | Celery seeds were less thermoinhibited when dried back after a seed soak treatment with the gibberellins A4 and A7 (GA4/7) plus ethephon (G+E) or an osmotic priming treatment in the light with polyethylene glycol (PEG). At temperatures between 18 and 25° in the dark, 50 percent of the PEG-treated seeds germinated after 3 days whereas G+E-treated seeds required 7 days and untreated seeds did not germinate at all.Irrespective of treatment, dry control and dried-back, treated seeds contained very little detectable cytokinin activity. However, when such seeds were imbibed for 18 h in the dark and then analysed immediately with the soybean callus bioassay, less cytokinin activity was detected in both G+E and PEG seeds than in the untreated seeds. In particular, cytokinins with the HPLC properties of zeatin and its riboside were decreased in G+E seeds and virtually absent from PEG seeds. Conversely, extracts from PEG and to a lesser extent G+E seeds contained activity which chromatographically resembled cytokinin glucosides whereas this was absent from untreated seeds. |
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Keywords: | Cytokinins celery seeds polyethylene glycol gibberellins ethephon |
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