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| 松茸液态发酵菌丝生长量因子的研究 | |
| 引用本文： | 李长田,刁盈盈,毛欣欣,李玉.松茸液态发酵菌丝生长量因子的研究[J].菌物学报,2012,31(2):229-234. |
| 作者姓名： | 李长田 刁盈盈 毛欣欣 李玉 |
| 作者单位： | 1. 吉林农业大学中药材学院 吉林长春130118;吉林农业大学食药用菌教育部工程研究中心 吉林长春130118 2. 吉林农业大学中药材学院 吉林长春130118 3. 吉林农业大学食药用菌教育部工程研究中心 吉林长春130118 |
| 基金项目： | 吉林省科技发展计划项目（No. 21200253） |
| 摘 要： | 研究了液态发酵条件下通气量、转速、pH、可溶性淀粉浓度对松茸菌丝生长量的影响，探讨了可溶性淀粉稳定pH的作用机理。试验结果表明：通气量≤0.4vvm时松茸菌丝产量随通气量的增大而增长，当通气量＞0.5vvm时，菌丝产量增加不明显；转速增大菌丝产量增加，转速＞80r/min，菌丝产量下降；松茸菌丝发酵的最适pH为5.5，初始pH不同，发酵罐中培养基最终的pH趋于6.0；可溶性淀粉是影响松茸菌丝量的重要因子，可溶性淀粉浓度≤4g/100mL时菌丝量随可溶性淀粉浓度的增加而增大，当其浓度为4g/100mL时，菌 |
| 关 键 词： | 通气量 转速 pH 可溶性淀粉 菌丝生物量 |
The affection factors of Tricholoma matsutake mycelial biomass in liquid fermentation |
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| Authors: | LI Chang-Tian DIAO Ying-Ying MAO Xin-Xin and LI Yu |
| Institution: | College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin 130118, China Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China;College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin 130118, China;College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China |
| Abstract: | The effects of aeration,agitation rate,pH value and soluble starch concentration on the biomass of Tricholoma matsutake in liquid fermentation were studied,and the possible mechanisms of soluble starch in maintaining the pH value are discussed.The results showed that the biomass of T.matsutake increased with the increase of aeration when aeration was at ≤0.4vvm,and increased slowly when the aeration was over 0.5vvm.The biomass of mycelium also increased with the increment of agitation rate but declined at 80r/min.The optimum pH is 5.5.With different initial pH value,the final medium pH tended to be 6.0.Soluble starch is an important factor influencing biomass of T.matsutake in fermentation.The biomass increased with the concentration of soluble starch when the concentration ≤4g/100mL.At the concentration of 4g/100mL,the biomass of T.matsutake reached 22g/L,and the mycelium yield could be increased by 91.3%. |
| Keywords: | aeration agitation rate pH soluble starch mycelium biomass |
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