Enhancement of catalytic activity of porcine pancreatic lipase by reductive alkylation |
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Authors: | T. N. B. Kaimal M. Saroja |
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Affiliation: | (1) Regional Research Laboratory (CSIR), 500 007 Hyderabad, India |
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Abstract: | Summary Chemical modification by reductive alkylation of porcine pancreatic lipase with n-butyraldehyde, isobutyraldehyde and acetone resulted in about 50% increase in theVmax of the enzyme.Eact was reduced by about 40% by this modification. Both hydrolytic and interesterification activities were enhanced without affecting the 1,3-specificity of the enzyme. |
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