Enhancement of catalytic activity of porcine pancreatic lipase by reductive alkylation

Summary Chemical modification by reductive alkylation of porcine pancreatic lipase with n-butyraldehyde, isobutyraldehyde and acetone resulted in about 50% increase in theV _max of the enzyme.E _act was reduced by about 40% by this modification. Both hydrolytic and interesterification activities were enhanced without affecting the 1,3-specificity of the enzyme.