脑细胞生长肽对GM耳毒性的预防作用

目的：观察脑细胞生长肽（cerebral cell growth peptide,CCGP)对庆大霉素（gentamicin,GM)引起的豚鼠耳中毒的预防作用）。方法：用脑干听觉诱发电位（brainstem auditory evoked potential,BAEP)和组织化学方法分别检测动物听阀的变化和耳蜗毛细胞线粒体内琥珀酸脱氢酶（succinate dehydrogenase,SDH)及溶酶体内的酸性磷酸酶（acid phosphatase,ACP)活性变化,并在三组动物进行耳蜗受损毛细胞计数。结果：CCGP能降低GM引起的BAEP反应阈的上升幅度,保护耳蜗毛细胞SDH和维持溶酶体的完整性,减轻毛细胞的损伤。结论：CCGP能降低GM的中毒性;保护耳蜗毛细胞SDH,维持毛细胞的能量代谢,减少溶酶体损伤,降低溶酶体内水解酶逸出引起的毛细胞自溶性损伤,可能是CCGP预防GM耳毒性的机制之一。

Preventive effects of cerebro cellular growth peptide on gentamycin-induced inner ear damage in guinea pigs

Aim:To investigate the preventive effects of the cerebrocellular growth peptide (CCGP) on gentamycin induced inner ear damage in guinea pigs,and to clarify its mechanism.Methods: The hypoacusis severity and enzymetic activity in the cochlear hair cells were examined by brainstem auditory evoked potential (BAEP) and histochemistry, respectively.The damaged hair cells was counted in three groups.Results: CCGP reduced the elevated BAEP reaction thresholds. It protected activities of mitochondrial succinate dehydrogenase and lysosome acid phosphatase in the cochlear hair cells.The number of damaged hair cells in the CCGP group was less than that in the gentamycin (GM) group. Conclusion: CCGP can reduce GM ototoxicity. The mechanism may be associated with the protective activity of mitochondrial enzyme,the maintenance of lysosome intactness, energy metabolism of the cochlear hair cells,and reduction of autolysis of hair cells induced by hydrolase overflowing from lysosome.