Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. |
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Authors: | M. C. Chen L. L. Chen Y. H. Lee C. Y. Wen W. S. Chu |
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Affiliation: | (1) Food Industry Research and Development Institute, P.O.Box 246, Hsinchu, Taiwan, R.O.C. |
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Abstract: | Summary Three screening methods were used to isolate GL-7-ACA acylase-producing strains. Three positive isolates were identified with Pseudomonas nitroreducens CCRC 11041 possessing the highest activity, against GL-7-ACA and GL-7-ADCA. No activity was detected when Ceph C or succinyl-7-ACA was used as substrate; glutaric acid was found to be inhibitory. CCRC 11041 could produce maximal GL-7-ACA acylase activity when cultivated on meat extract medium II. The enzyme had a pH optimum of 5.0 and a temperature optimum of 42°C. |
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