Synthesis of marimastat and a marimastat conjugate for affinity chromatography and surface plasmon resonance studies |
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Authors: | Jenssen Kai Sewald Katherina Sewald Norbert |
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Institution: | Department of Chemistry, University of Bielefeld, Universit?tsstrasse 25, D-33615 Bielefeld, Germany. |
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Abstract: | Immobilized small-molecule inhibitors are suited for enrichment of biomolecules by affinity chromatography, as it is shown for metalloproteinases and an immobilizable derivative of the hydroxamate-type inhibitor marimastat. A new asymmetric synthesis of marimastat is presented that allows for site-specific attachment to a solid surface, e.g., a chromatography matrix or a surface plasmon resonance sensor chip. The latter technique is shown to be a valuable tool for the optimization of binding and elution conditons of biomolecules in affinity chromatography. |
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