The effect of oxygen on the sporulation and toxicity of Bacillus sphaericus 2362 |
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Authors: | Mohamed Ismail A Karim Rachel J Lucas Keith J Osborne Peter L Rogers |
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Affiliation: | (1) Faculty of Food Science & Biotechnology, Universiti Pertanian Malaysia, 43400 UPM Serdang, Malaysia;(2) Department of Biotechnology, University of New South Wales, PO Box 1, 2033 Kensington, NSW, Australia |
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Abstract: | Summary A detailed kinetic study of the effect of DO levels on the growth and rate of sporulation of Bacillus sphaericus 2362 is reported. It was found that use of DO levels of 20%, 50% and 100% gave rise to rapid formation of free spores, whilst use of 5% DO slowed this rate approximately eight-fold. In addition, toxicity of the resultant spore preparations was determined. The preparations from cells grown at 5%, 20% and 50% DO gave comparable levels of mosquito-larvicidal activity, whilst that from the cells grown at 100% DO was significantly less toxic. |
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