Orinithine as a constituent of the peptidoglycan of Chloroflexus aurantiacus,diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum |
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Authors: | U J Jürgens J Meißner U Fischer W A König J Weckesser |
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Institution: | (1) Institut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität, Schänzlestr. 1, D-7800 Freiburg i. Br., Federal Republic of Germany;(2) Fachbereich Biologie, AG Geomikrobiologie der Universität Oldenburg, Postfach 2503, D-2900 Oldenburg, Federal Republic of Germany;(3) Institut für Organische Chemie, Martin-Luther-King-Platz 6, D-2000 Hamburg 13, Federal Republic of Germany |
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Abstract: | L-Ornithine is the only diamino acid of the peptidoglycan of the gliding phototrophic Chloroflexus aurantiacus. The other constituents are L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (in part as muramic acid-6-phosphate), all in approximate equimolar ratios to L-ornithine, aside from small amounts of glycine and histidine. Furthermore unlike typical Gram-negative bacteria, protein is not bound to this peptidoglycan. Instead, the rigid layer (sodium dodecyl sulfate insoluble cell wall fraction) contained large amounts of a complex polysaccharide consisting of sugar O-methyl ethers, hexoses and pentoses. Its binding site is presumably muramic acid-6-phosphate of the peptidoglycan.In contrast, in Chlorobium vibrioforme f. thiosulfatophilium, meso-diaminopimelic acid was found as the only diamino acid of this peptidoglycan. As with other Gramnegative bacteria, L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (no muramic acid-6-phosphate) were observed in approximate equimolar ratios to meso-diaminopimelic acid, except a lower D-alanine content. The rigid layer of Chlorobium vibrioforme f. thiosulfatophilum contained protein, and there were no indications for a complex polysaccharide comparable to that of Chloroflexus aurantiacus.Abbreviations Ala
alanine
- A2pm
diaminopimelic acid
- GC/MS
combined gas-liquid chromatography/mass spectrometry
- GlcNAc
N-acetyl-glucosamine
- Glu
glutamic acid
- Gly
glycine
- HF
hydrofluoric acid
- Lys
lysine
- MurNAc
N-acetyl-muramic acid
- Orn
ornithine
- SDS
sodium dodecyl sulfate |
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Keywords: | Chlorobium Chloroflexus Cell wall Diaminopimelic acid Muramic acid-6-phosphate Ornithine Peptidoglycan-polysaccharide complex |
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