Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strain. |
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| Authors: | K Hirose M Fujita M Takeuchi N Yumoto M Tokushige Y Kawata |
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| Affiliation: | Department of Chemistry, Faculty of Science, Kyoto University, Japan. |
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| Abstract: | We have cloned the structural gene (tdcB) of biodegradative threonine deaminase from Escherichia coli W strain by utilizing the polymerase chain reaction. The JM109/pUCTDA strain, which was obtained by transforming E. coli JM109 with a vector plasmid (pUCTDA) containing the cloned tdcB gene, produced a large amount of the enzyme corresponding to more than 5% of the total soluble protein. Amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to the nucleotide-deduced sequence of biodegradative threonine deaminase from E. coli K-12. |
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