A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues. |
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Authors: | H P Bennett A M Hudson L Kelly C McMartin and G E Purdon |
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Abstract: | Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography. |
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