Cell division and nucleolar ribonucleic acid synthesis in synchronous cultures |
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Authors: | Edwin V Gaffney Roland M Nardone |
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Institution: | (1) Department of Biology, The Pennsylvania State University, 16802 University Park, Pennsylvania;(2) Department of Biology, The Catholic University of America, Washington, D.C. |
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Abstract: | Summary Nucleolar RNA synthesis is inhibited and cell division delayed in synchronous cultures of mouse fibroblasts (strain L-929)
treated with actinomycin D (0.04 μg per ml). The gradual loss of actinomycin D from the cells during a 2-hr period following
incubation is accompanied by an increase in the rate of nucleolar RNA synthesis to the control level. Following this the rate
of protein synthesis is decreased by 25% for approximately 9 hr. The length of time that nucleolar RNA and protein synthesis
are inhibited accounts for the delay in mitosis 1 1/2 cell cycles later. These data support the contention that certain proteins
produced during one interphase are prerequisite for division in a subsequent cycle. |
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