Echinococcus granulosus and E. multilocularis: soluble antigen fluorescent antibody test |
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| Authors: | R W Gore E H Sadun R Hoff |
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| Affiliation: | 1. Guangdong Provincial Key Laboratory for Processing and Forming of Advanced Metallic Materials, South China University of Technology, Guangzhou 510640, China;2. National Engineering Research Center of Near-net-shape Forming for Metallic Materials, South China University of Technology, Guangzhou 510640, China;1. Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea;2. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-gun, Jeollanam-do 58545, Republic of Korea;3. Agency for Korea National Food Cluster (AnFC), Iksan 54576, Republic of Korea;4. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea;1. Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;2. Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA |
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| Abstract: | A soluble antigen fluorescent antibody test for the laboratory diagnosis of human echinococcosis is described. A satisfactory degree of sensitivity, specificity, and reproducibility was obtained in this test when an antigen extracted from scolices and fractionated by Chromatographic methods was employed. Conversely, fractions of hydatid fluid obtained in the same manner did not permit a desirable degree of specificity, since a large percentage of sera from individuals with parasitic, bacterial, and mycotic infections reacted in the test. Antigen extracted from Echinococcus granulosus scolices permitted the detection of E. multilocularis infections as well. This technique may be suited as a screening procedure in investigating the seroepidemiology of hydatid disease and for the mass screening of domestic animals in highly endemic areas. |
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