Depleted uranium is not toxic to rat brain endothelial (RBE4) cells |
| |
| Authors: | Allison W Dobson Anna K Lack Keith M Erikson Michael Aschner |
| |
| Institution: | (1) Department of Life Sciences, Winston-Salem State University, Winston-Salem, NC;(2) Department of Physiology and Pharmacology, Wake Forest University Medical Center, Winston-Salem, NC;(3) Department of Nutrition, University of North Carolina, Greensboro, NC;(4) Department of Pediatrics and Pharmacology, Vanderbilt University Medical Center, Nashville, TN;(5) Kennedy Center, Vanderbilt University Medical Center, Nashville, TN |
| |
| Abstract: | Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of
possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the
determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat
shock protein 90 (Hsp90) expression, 3-4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate
dehydrogenase (LDH) activity. The results of these studies show that uptake of the U3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these
cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication
for overt cytotoxicity in endothelial cells exposed to DU. |
| |
| Keywords: | Depleted uranium (DU) heavey metal toxicity blood-brain barrier endothelium |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
